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|Title: ||The Roles of daf-6 and Cell-Cell Interactions in Sensory Organ Morphogenesis|
|Authors: ||Perens, Elliot A.|
|Keywords: ||sensory organ morphogenesis|
|Issue Date: ||2006|
|Abstract: ||The development of multicellular organs depends on the regulation of cell shape, position, and orientation. The genetic regulation of these morphogenetic processes is poorly understood. As a model for organ morphogenesis, I studied
the development of the Caenorhabditis elegans amphid sensory organ. Sensory
organs in diverse species are often composed of neuronal sensory endings
accommodated in a lumen formed by ensheathing epithelia or glia. The
generation of this structure may require cell-autonomous factors that control
lumen formation, as well as cell non-autonomous factors that coordinate the
morphogenesis of the lumen with the resident neuronal processes.
Understanding these processes would provide insight into lumen formation, glia
morphogenesis, and cell-cell interactions during development, especially neuronal regulation of glia morphogenesis.
In this thesis, I identify and characterize genes required for lumen formation in the amphid sensory organ. First, the gene daf-6 is required cell autonomously during amphid lumen formation. daf-6 encodes a Patched-related protein that is a member of a previously uncharacterized sub-family of sterolsensing domain containing proteins. Interestingly, daf-6 is expressed and
required in several tubular structures, such as the excretory system and vulva.
Thus, a similar genetic pathway is required for the formation of different lumens.
Secondly, I conducted a forward genetic screen and identified and characterized
mutations that suppress the lumen formation defects in daf-6 mutants. Finally,
by examining mutants defective in sensory neuron process formation, I showed
that amphid lumen shape is determined by its resident sensory endings.|
|Description: ||A thesis presented to the faculty of The Rockefeller University in partial fulfillment of the requirements for the degree of Doctor of Philosophy.|
|RU Department Name: ||Shaham Laboratory|
|Appears in Collections:||Student Theses|
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